LysM-GFP monocyte-neutrophil reporter mice [4] received 20 μg goat anti-CCL2 IgG, 20 μg goat anti-CCL7, or 20 μg goat IgG by intraperitoneal injection at − 12, 0, and + 12 h relative to time of infection. Here, CCL2 is linked to infection.