We comprehensively evaluated the serum levels of macrophage-related markers (IL-34, M-CSF, soluble CD163), 40 cytokines/chemokines, hyaluronic acid, type IV collagen 7s, and clinically approved fibrosis scores, such as aspartate aminotransferase to platelet ratio index (APRI), fibrosis 4 (FIB-4) index, NAFLD fibrosis score. Here, IL34 is linked to metabolic dysfunction-associated steatotic liver disease.