PLK1 and neoplasm: The inhibition of Plk1 expression can cause apoptosis of tumor cells, which provides a good strategy for tumor therapy.23, 24, 25 Thus, LGCP was designed to bring the powerful gene editing toolbox (Cas9 protein/sgPlk1 plasmid, here “sgPlk1 plasmid” represents the plasmid encoding only the single guide RNA, without Cas9 in this paper) into tumor cells and tissues to inhibit tumor progression with high efficiency.