There was a significant increase for 3D cultured cancer cells in the protein expression of key pro-angiogenic molecules, such as VEGF-A (3D: 2.313 ± 0.110 vs. 2D: 1.124 ± 0.111, P < 0.01) (Fig. 3a, b) and bFGF (3D: 2.687 ± 0.086 vs. 2D: 1.999 ± 0.077, P < 0.01) (Fig. 3a, c). This evidence concerns the gene FGF2 and cancer.