Additionally, we found that the localization of these proteins was different: Vimentin was expressed in the tumour stroma, AGR2 was located in the cytoplasm of cancer cells, CD44 was mainly located in cancer cells membrane and partially in the cytoplasm, Slug and SOX2 were expressed in the nucleus of tumour cells, but KLF4, ALDH1 and C4.4A were expressed in the cytoplasm and nucleus of cancer cells. The gene discussed is VIM; the disease is cancer.