Cluster analysis about mRNA expression between TRAF6 and some common EMT and CSC markers has been operated; result showed that TRAF6 and EMT and CSC mRNAs in SCCHN are correlated (Fig. S1B), especially a strong correlation with CD44. Tgfbr1 and Pten knockout mice with de novo SCCHN tumorigenesis have been reported in our previous study 38. The gene discussed is TGFBR1; the disease is head and neck squamous cell carcinoma.