The analysis results indicated that the original SCCHN with lymph node metastasis status (N1 + N2, n = 42, P < 0.05) had stronger immunoreactivity than the original SCCHN with lymph node‐negative status (N0, n = 22, Fig. 1B and E), and the expression of TRAF6 was still located in the cytoplasm and partially in the nucleus of cancer cells. The gene discussed is TRAF6; the disease is cancer.