To observe Cas-3-specific generation of azido (N3) groups by Apo-S-Ac3ManNAz, we additionally estimated bioorthogonal apoptosis tracking efficacy of Apo-S-Ac3ManNAz using both LNCaP (human prostate carcinoma) cell line and HT-29 (human colorectal adenocarcinoma) cell line. Here, AOPEP is linked to prostate carcinoma.