2001; Park et al. 2014; Miszczuk et al. 2015) and TLC‐induced retrieval of PM‐MRP2 required activation of PKCε followed by MARCKS phosphorylation (Schonhoff et al. 2013). The present study shows that cAMP and TUDC inhibit TLC‐induced increases in PM‐PKCε and MARCKS phosphorylation in both hepatocellular carcinoma cell line and primary hepatocytes. These results are consistent with the hypothesis that cAMP and TUDC can reverse TLC‐induced PM‐MRP2 retrieval by inhibiting TLC/PKCε/MARCKS phosphorylation pathway. This evidence concerns the gene PRKCE and hepatocellular carcinoma.