To investigate if miRNAs are involved in regulating PLCE1 expression in ESCC, three online databases, namely, TargetScan, miRanda, and miRDB, were used to predict the miRNA that can target the PLCE1 of the miRNA; miR-34a gained our attention because it is a well-known anti-oncogene, and the databases predicted miR-34a as a potential miRNA that targets PLCE1. The gene discussed is PLCE1; the disease is esophageal squamous cell carcinoma.