In order to further explore the role of PTTG1 and GLI1 in the invasion and metastasis of ESCC, we detected the expression levels of PTTG1 and GLI1 in three ESCC cell lines with different metastasis ability and immortalized human esophageal epithelial cell line SHEE. This evidence concerns the gene PTTG1 and esophageal squamous cell carcinoma.