To determine the contribution of lncRNA H19 to chemoresistance in breast cancer cells, a cell line, MCF-7/Dox1600/shH19, was established by stable transfection of a specific H19 shRNA expression vector in MCF-7/Dox1600 cells to knock down H19 expression as described in the Materials and Methods. This evidence concerns the gene H19 and breast carcinoma.