VIM and metastatic malignant neoplasm in the lymph nodes: During EMT, Vimentin upregulation promotes the gain of functional and morphological mesenchymal cell characteristics in epithelial cells and drives the cellular architecture toward a migratory and invasive phenotype.46 The Vimentin expression level was increased in GBC specimens and is significantly higher in metastases than in primary tumors, which indicates that Vimentin is associated with GBC metastasis and lymph node metastases.27,47