S100 calcium binding protein A4 (S100A4) promotes EMT by inducing mesenchymal traits in tumors51 and is indicative of tumor progression and metastasis.52 As a mesenchymal EMT marker, excessive S100A4 expression was inversely correlated with the loss of E-cadherin in GBC21 and was associated with poor GBC patient survival.26,53 However, its clinical value with regard to clinicopathological factors is controversial. This evidence concerns the gene S100A4 and neoplasm.