Here, we demonstrate that the amount of both ATG5 and LC3B-II was markedly increased in 3AO and SKOV3 spheres, concurrent with increased degradation of the autophagy cargo protein SQSTM1, in the presence of BafA1 and in the context of starvation induced by EBSS treatment, compared with bulk ovarian cancer cells. Here, SQSTM1 is linked to ovarian cancer.