We then infected B16F10 cells, a melanin-producing mouse melanoma cell line, with lentiviruses carrying control shRNA or Jmjd6 shRNA, and/or infected with retroviruses carrying vector or expression plasmids for PAK1, PAK1Δ15, JMJD6, or JMJD6m. Here, JMJD6 is linked to melanoma.