JMJD6 and melanoma: To substantiate this notion, transwell invasion assays showed that JMJD6 depletion resulted in a decrease in the invasive potential of the highly invasive SK-MEL-1 melanoma cells, an effect that could be offset by simultaneous overexpression of PAK1, but not PAK1Δ15, whereas overexpression of JMJD6, but not JMJD6m, led to an increase in the invasive potential of cells (Fig. 4d).