The obese AT microenvironment was recapitulated using a co-culture cellular ratio of 10% CD8+ T cells to adipocytes, as described in obese AT by Nishimura and colleagues [22], which was stimulated with LPS at a concentration that mimics in vivo circulating levels in obesity [83,109,254]. The gene discussed is CD8A; the disease is obesity due to melanocortin 4 receptor deficiency.