BECN1 and Stroke: Western blotting for LC3 and Beclin1 in ischemic brain tissues at 6 h, 1 day, 3 days, and 7 days post stroke demonstrated that the ratio of LC3-II/LC-I and the expression of Beclin1 increase at 6 h post ischemia and reach their peaks at 3 days post stroke (Fig 3C and 3D).