TNF and Alzheimer disease: In cortical homogenates only, we analyzed the expression of the presynaptic marker synaptophysin by Western blotting (WB) and measured levels of pro-inflammatory cytokines TNFα, IL-1β, IL-6, CXCL10 and CCL2 by bead-based multiplex assay (Luminex) to determine whether the irradiation aggravates synaptic and neuro-inflammatory hallmarks of AD.