A study by De Wilt et al. in lung cancer cells with acquired BTZ resistance revealed Ala49Thr, Met45Val, and Cys52Phe substitutions in the β5 subunit BTZ-binding pocket, while Suzuki et al., in BTZ-resistant colon cancer cells observed Cys63Phe and Arg24Cys mutations in the β5 subunit [29, 148]. The gene discussed is CASC3; the disease is colonic neoplasm.