We used chromatin immunoprecipitation qPCR (ChIP-qPCR) to examine the binding of Notch1 to the promoter of Rheb and Hes1 (as a positive control) in angiomyolipoma cells in Dulbecco’s modified Eagle’s medium (DMEM) to identify which sites are functionally important and correspond to true NREs. The gene discussed is RHEB; the disease is angiomyolipoma.