The cell-specific depletion of Tsc1 in nestin-expressing kidney cells in adult Nestin-TamCre;Tsc1f/f mice resulted in multiple bilateral cysts, renal intraepithelial neoplasia (RIN), and invasive multifocal papillary carcinomas composed of eosinophilic tumor cells (Fig. 7b, c), consistent with morphology observed in TSC-associated RCCs60. This evidence concerns the gene NES and neoplasm.