The loss of nestin in angiomyolipoma cells during the formation of sphere-like structures (Fig. 3b, c(i), e, f(i)) correlated with the adoption of an early neuronal or melanocyte fate, indicated by increases in percentages of NS-tubulin-positive cells (Fig. 3b, c(ii), e, and f(ii)) and cells co-expressing NS-tubulin and melan-A (Supplementary Fig. 5E), respectively, suggests neuronal traits. The gene discussed is NES; the disease is angiomyolipoma.