We further investigated the relationship between ALX4 expression and clinical parameters, and found that ALX4 expression was evidently correlated to clinical stages (n = 140, P = 0.01), tumor size (n = 142, P = 0.01) and lymph node status (n = 138, P = 0.00) of breast cancer patients (Table 1). This evidence concerns the gene ALX4 and breast carcinoma.