Ten other genes had p value of 0.0001 in the unadjusted differential expression analysis including two with decreasing gene expression levels in more advanced CRC stages (RNF34 and NUDT6) and eight with increasing gene expression levels in more advanced CRC stages (LRCH4, HIST3H2BB, SPSB3, HIST2H4A, TMEM79, AMIGO2, GLB1L and C20orf135) (Table 2, Fig 1). This evidence concerns the gene SPSB3 and colorectal carcinoma.