Although allergic mice treated with L-CE caused reduction of airway remodeling and inflammatory cytokine response as well as increased Th1 IFN-γ expression, the vaccine caused increased inflammatory cell infiltration into the lungs after allergen provocation [which might be due to the pathogen associated molecular patterns (PAMPs) including endotoxin and/or unknown impurity in the preparation] and failed to generate the regulatory responses which are the most effective immunological features in controlling the allergic diseases. The gene discussed is IFNG; the disease is allergic disease.