To test the utility of NSC as a source of analyzable miRNAs, we chose two miRNAs involved on insulin secretion signaling as well as obesity and T2D: miR-33b, which has been associated with glucose and lipid metabolism and insulin signaling [10,11,12] and miR-375, which has been found to be altered in induced diabetes and associated with the regulation of murine adipogenesis and insulin secretion [13,14,15]. The gene discussed is INS; the disease is Obesity.