ATP5MC2 and colorectal carcinoma: We determined the length of the uc.339 transcript by rapid amplification of cDNA ends (RACE), and we assessed the endogenous expression of uc.339 in three NSCLC cell lines (A549, H1299, and H460) and one CRC cell line (LoVo) by QuantStudio 3D digital PCR (dPCR), carefully selecting for primers designed to amplify within the ultraconserved area as described by Bejerano et al.1, but not overlapping with the ATP5G2 host gene (Fig. 1c).