To validate the enhanced induction of apoptosis by 5-aza-dC in combination with IR in pancreatic cancer cells, we investigated whether the combination of 5-aza-dC and IR (2, 4, and 8 Gy) could regulate cell apoptosis by performing flow cytometry, caspase 3 and 7 activity assays, and comet assays. The gene discussed is CASP3; the disease is familial pancreatic carcinoma.