In our analysis, although the percentage of CD44+ subpopulations of MIA PaCa-2 or PANC-1 cells isolated from tumor spheres was lower in the groups treated with the combination of 5-aza-dC and IR, the percentage of CD44- subpopulations, which lack stem cell-like properties, was significantly higher than that in the groups treated with 5-aza-dC or IR alone or the control groups (Figure 5A and 5B). This evidence concerns the gene CD44 and neoplasm.