To further dissect the tumor suppressive function of ANKRD1 in the p53 pathway, we utilized the colon cancer cell line HCT116 that harbors a homozygote deletion of the TP53 locus (HCT116 TP53−/−) and the corresponding HCT116 wild type (HCT116 TP53+/+) [40]. Here, TP53 is linked to colonic neoplasm.