WWC1 and hepatocellular carcinoma: We analyzed the methylation levels of core Hippo components such as KIBRA, RASSF1A, MST1, MST2, WW45, LATS1, LATS2 and YAP1 in five matched primary tumors (hepatocellular carcinoma) and normal liver samples by combined bisulfite restriction analysis (Supplementary Figure 4).