CFTR and cystic fibrosis: 2007). The sequencing method applied in our study allowed to find 70 sequence variations. Eight of these are CF‐causing mutations: p.F508del, p.G85E, p.G330E, p.A455E, p.G970S, W1098X, R1162X, and N1303K and probably p.H609R. The mutation detection rate of the methodology in this study is 81% in patients with confirmed diagnosis of CF. It has been demonstrated that CFTR gene sequencing has greater sensitivity relative to other molecular techniques such as hybridization, SSCP, DDGE (Dequeker et al. 2009).