TRIB1 and hepatocellular carcinoma: Western blot and qRT-PCR analysis of TRIB1 and p53 expression in the HCC cell lines SMMC-7721(wide type p53), HepG2(wide type p53), and Huh7(mutant p53 Y220C) and the normal liver cell line LO2 showed that TRIB1 expression was significantly higher and p53 expression was significantly lower in HCC cells than in normal liver cells (Figures 1D,E).