IL10 and infection: On the other hand, the specific IFN-γ response generated by TLA increased tendentiously comparing primed with non-primed animals, and was 5 fold higher than the TLA induced IL-10 response (Fig 6ii), making this vaccination approach interesting to be tested in terms of anti-Leishmania effectiveness, in the context of natural infection (parasites delivered by the sand fly in the presence of salivary proteins).