For this analysis we used the same blood draw of 7/14 T1D patients and 6/14 healthy donors to isolate CD4+ T cells specific for the T1D autoantigen glutamic acid decarboxylase (GAD65) and for cytomegalovirus (CMV) after stimulation in vitro26–28 and obtain TCRB sequences from single cells29. The gene discussed is GAD2; the disease is type 1 diabetes mellitus.