For this analysis we used the same blood draw of 7/14 T1D patients and 6/14 healthy donors to isolate CD4+ T cells specific for the T1D autoantigen glutamic acid decarboxylase (GAD65) and for cytomegalovirus (CMV) after stimulation in vitro26–28 and obtain TCRB sequences from single cells29. Here, CD4 is linked to type 1 diabetes mellitus.