Finally, we tested whether the formation of droplets could be induced in RCC4 VHL cells when the cells were cultured under hypoxic conditions, and found hypoxia could potently induce lipid deposition in the presence or absence of LA/OA (Fig. 1f), suggesting a model that by regulating the hypoxia pathway, VHL negatively regulates lipid accumulation in renal cancer cells. Here, VHL is linked to renal carcinoma.