Taken together, NGF-induced Mart-1:GFP-positive cells showed gene expression changes similar to C81-61 non-metastatic melanoma cells in contrast to RA-induced Mart-1:GFP-positive melanoma cells that showed no overall gene expression changes from wild-type C8161 melanoma cells, and only a transient spike in Mart-1:GFP expression, indicating RA turns on the Mart-1:GFP reporter but is insufficient to drive Mart-1 protein expression. Here, NGF is linked to melanoma.