NGF and melanoma: After co-culturing C8161 metastatic melanoma cells with NGF for 72 h, we isolated Mart-1:GFP-positive re-expressing C8161 cells by FACS and compared gene expression profiles to wild-type C8161 and C81-61 cells using qPCR (using C8161 as baseline expression, light blue bar; Fig. 6A).