ELISPOT results (Figures 4A and 4B) showed that K562, an NK cell-sensitive leukemia cell line, was efficient in stimulating IFN-γ secretion by PBC-iPSC#9-NK cells, whereas Raji, an NK cell-resistant lymphoma cell line, was less efficient, suggesting that PBC-iPSC-NK cells are capable of secreting cytokine in response to stimulation. This evidence concerns the gene IFNG and leukemia.