We detected seven variants above the call threshold including a BRAF V600E mutation, which has been described in cholangiocarcinoma with poor prognosis.43 Importantly, the same seven variants were found in the original human tumor sample and the derived cell culture subclones (http://onlinelibrary.wiley.com/doi/10.1002/hep.29679/suppinfo). This evidence concerns the gene BRAF and cholangiocarcinoma.