If needed, sensitivity could be improved further by stably transfecting into the NanoLuc-VAMP2 SiMa neuroblastoma cells the mouse synaptotagmin 2 which has 10 times higher affinity to BoNT/B compared to human synaptotagmin (Strotmeier et al., 2012). The gene discussed is SYT2; the disease is neuroblastoma.