Mice with established tumors (~400 mm3) were injected intravenously with CD8a-targeting nanoparticles, and immune cells were recovered from the circulation, spleen, tumor, and tumor-draining lymph node over a timeframe of 48 h (Fig. 2c, gating strategy shown for 24 h in Supplementary Fig. 2a). This evidence concerns the gene CD8A and neoplasm.