NOTCH1 and esophageal squamous cell carcinoma: Moreover, the ability of esophageal neoplastic cells to form single-cell-derived 3D organoids was attenuated upon Cre-mediated ex vivo Notch1 deletion in single-cell suspensions prepared from dysplastic lesions or ESCC tumors of 4NQO-treated Notch1loxP/loxp mice (Fig. 2f).