While these data support a role for Notch1 in promotion of ESCC tumor cells with attributes of EMT, EpCAMneg cells were rare in cultured TE11 cells and ectopic ICN1 expression alone had no influence upon this cell population (Supplementary Fig. 2c), indicating that Notch1 activation alone may not be sufficient to drive EMT. Here, NOTCH1 is linked to esophageal squamous cell carcinoma.