Hence, to examine the kinetics of hypoxia-induced changes of CFTR protein and mRNA levels, we performed time-course studies during physiological hypoxia in two lung epithelial cell lines, Calu3 cells (epithelial lung adenocarcinoma; derived from metastatic site: pleural effusion) and 16HBE14o- cells (SV40-immortalized human bronchial epithelial cells). The gene discussed is CFTR; the disease is Pleural effusion.