Figure 4B shows that inhibition of NOS activity with L-NAME (100 μM) had no effect on evoked [3H]ACh release from the LM-MP of the ileum of control and TNBS-treated rats, indicating that NO does not mediate inhibition of [3H]ACh release in TNBS-induced ileitis. The gene discussed is NOS1; the disease is Crohn ileitis.