In view of the prominent role played by both the spleen and α7 nAChR signaling in mediating the CAP, we asked whether the protection afforded by AR-R17779 against TNBS-induced colitis could be maintained also after SPX and we demonstrated that, in our model of experimental colitis, the presence of the spleen is essential to the anti-inflammatory effect of exogenous α7 nAChR stimulation. Here, CHRNA7 is linked to colitis.