To identify tumor cells with elevated EGFR expression in heterogeneous GBMs and select suitable models for evaluating targeting efficiency, the human GBM cell lines U-87MG, U-251, and DBTRG-05MG were used for evaluating the EGFR expression profile through Western blotting, flow cytometry, and IF staining. The gene discussed is EGFR; the disease is glioblastoma.