Both ramified and amoeboid Iba1 positively stained cells were identified, with amoeboid cells that could be either microglia or perivascular macrophages (17) mostly associated closely with the 4T1-GFP foci, while ramified microglia were found both close to the tumor foci and further away in the broader tumor microenvironment (Figure 1A). This evidence concerns the gene AIF1 and neoplasm.