In another study, Ilie et al. evaluated PD-L1 IHC expression in 160 paired biopsy and resected specimen of NSCLC (33 squamous cell carcinomas and 127 adenocarcinomas) using SP142 clone (Ventana, Roche, Tucson, AZ); at ≥ 1% cutoff (considering PD-L1 expression on tumor cell only), the authors found positivity in 23% of resection specimen versus 7% positive biopsies with an overall discordance rate of 19% and a κ value of 0.396 (poor agreement) between resection specimen and biopsies [16]. This evidence concerns the gene CD274 and non-small cell lung carcinoma.