SERPINE1 and ovarian cancer: After treatment with 10 μM IMD-4482 for 24 hours, the PAI-1 positive ovarian cancer cell lines (SKOV3ip1 and HeyA8) displayed increased catalytic activity, as shown by a white band indicating 33 kDa (low-molecular-weight uPA), while the PAI-1-negative ovarian cancer cell line (OVCAR3) showed no detectable changes.