Employing a comprehensive tissue microarray (TMA; n = 483) of human GBCs as well as a panel of eight biliary tract cancer (BTC) cell lines for xenograft and in vitro investigations, we here show that IMP2 expression, but not IMP1 and IMP3 expression correlates with poor prognosis and in vivo xenograft tumor growth. This evidence concerns the gene IGF2BP2 and biliary tract neoplasm.