GSEA shows the differential functional profiles between the clusters: the IA-like cluster is enriched in cancer-promoting signatures relating to immune response, NF-kB activation, pro-angiogenic signaling and proliferative pathways, whilst the IO-like cluster is enriched in signatures of chromatin remodeling and normal brain development (Figure 4B and Supplementary Table 7). The gene discussed is NFKB1; the disease is cancer.