The comparison of HEC number and HEC ratio among the three groups showed the expansion of CD4+ group is significantly less expanded than CD8+ (p < 0.0278 and p < 0.0179 respectively) and is also significantly less expanded than sarcoidosis tissue group (p < 0.0012 and p < 0.0034 respectively), while the CD4+ group is least expanded and the tissue group is the most expanded group (Figure 2). This evidence concerns the gene CD4 and sarcoidosis.