We focused our analysis on five mitochondrial regions near the origin of replication and transcription start site for the heavy strand: the displacement Loop (D-Loop), tRNA phenylalanine and 12S ribosome (tRNA-F+12S), 16S ribosome (16S), NADH dehydrogenase 5 (ND5) and cytochrome b (CYTB) mRNA-encoding gene (sequencing primers are shown in Table 1) in two human cell lines; In human myotubes and the ovarian cancer cell line SKOV3. Here, MT-CYB is linked to ovarian carcinoma.