To investigate how iNKT cells exert inhibitory roles toward NK cells and promote ethanol-induced liver injury and steatosis, the mRNA levels of various potential cytokines, including transforming growth factor-β (TGF-β), IL-4, and IL-10, in liver tissues and sorted hepatic iNKT cells were evaluated at different time points after the gavage of WT mice undergoing chronic-binge model. Here, IL4 is linked to steatosis.