In the present study, we demonstrated that NK cells play essential protective roles against liver steatosis through the IFN-γ-induced downregulation of fat metabolism-associated genes in hepatocytes in a chronic plus single-binge ethanol consumption mouse model, which can be inhibited by a IL-10-producing iNKT cell subset (also called NKT10 cells), suggesting an interplay between NK cells and NKT10 cells during ALD pathogenesis. Here, IL10 is linked to Hepatic steatosis.