ATG5 and Familial prostate cancer: Of note, FQ also caused an increase in LC3-II levels in prostate cancer PC3 and PC3M cells but not in DU-145 cells lacking full-length autophagy-related gene 5 (ATG5) (which is essential for LC3 lipidation), suggesting the necessity of the functional autophagic lipidation machinery (Supplementary Figure S3B).