Next, we examined β-catenin, PKM2 and miR-152 expression levels in 25 pairs of breast cancer specimens and their matched adjacent normal breast tissues (NBT), including 9 triple negative breast cancer (TNBC) tissues and 16 triple positive breast cancer (TPBC) tissues by using In situ hybridization and Immunohistochemical staining (Fig. 6B). Here, PKM is linked to breast cancer.