PKM and breast carcinoma: Next, we examined β-catenin, PKM2 and miR-152 expression levels in 25 pairs of breast cancer specimens and their matched adjacent normal breast tissues (NBT), including 9 triple negative breast cancer (TNBC) tissues and 16 triple positive breast cancer (TPBC) tissues by using In situ hybridization and Immunohistochemical staining (Fig. 6B).